Development of distributed HIS architecture based on C/S structure / 医疗卫生装备

Objective To develop a HIS architecture based on.NET in C/S environment,so that the different functional modules of the HIS can be easily loaded into the architecture to improve the efficiency of HIS development and reduce development costs.Methods The proposed HIS architecture underwent hierarchical structure design,and was composed of boundary,logic,data access and physical layers.The user input data through the boundary layer,responded to the user request through the logic layer,and reacted to the request from the logic layer through the data access layer to realize database access.The boundary,logic and data access layers transferred data through the physical layer which was used as the data carrier.C# language of Visual Studio 2008 was applied to the development of the architecture program.Results The system could conveniently load each function module in HIS of the hospital,which greatly facilitated the developer to control the function of the program.Conclusion The system meets the requirements of medical staff and management to the software,improves the efficiency of HIS system development,and reduces the cost of system development.

Isolation, purification and characterization of lectin from Enteromorpha intestinalis / 中国海洋药物

Lectin from green marine algae Enteromorpha intestinalis (EIL) was isolated by extraction with PBS, followed by ammonium sulfate fraction and a combination of ion-exchange chromatography on DEAE-52 fiber and gel filtration on SephadexG-200. Lectin showed single band on both PAGE and isoelectrofocusing electrophoresis. Its isoelectric point was 8.9 and molecular weight was 17783 tested on SephadexG-200. EIL displayed some agglutination activities with Chlamydomonas and erythrocytes of rabbit, common carp, crucian carp and human (A, B, AB, O), but the maximum agglutination was with crucian carp. The hemagglutinating activity for rabbit erythrocyte could not be inhibited by D-fructose, D-galactose, glucose, sucrose, mannose, lactose, bovine-thyroglobulin, egg albumin and gamma-globulin. The hemagglutinating activity of lectin would be showed at pH 4.0~10.2, and more potent at pH 6.9~8.0. The hemagglutinating activity was not affected at 90℃ for 1h, so it could be said that the lectin had high heat resistance.